Proteins that have low abundance in endogenous conditions.Novel or newly discovered proteins without the need to generate antibodies.Proteins whose antibodies are not available.Epitope tagging is an efficient way to study the following scenarios: Known genes fused to epitope tags can be easily detected or purified using well-characterized tag antibodies. Thereafter, the epitope-tagged fusion protein can be either detected or purified using an antibody specific for the epitope tag. The fusion gene is cloned in an expression vector appropriate for the desired host cell type and transfected. Epitope Tag Antibodies in Immunoprecipitation and Chromatin ImmunoprecipitationĮpitope tagging was first described by Munro & Pelham in 1984 as a technique where a known epitope is fused to a recombinant protein, often on the N- or C-terminus, using genetic engineering.Epitope Tag Antibodies in Immunofluorescence.Overview of Alzheimer’s Disease Antibody Targets.
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